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Feedback Contents Links,Assig'm MainPage Glossary GenLabOnLine GeneGateway Image Disclaimer Announcements BlackBoard MessageBoard
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Family
History Tool What
is in your family history?
Learn how to create your own
family tree and find out
| Human Genome |
| Explore human genome resources or browse the human genome sequence using the Map Viewer. |
http://www.genome.gov/10001772
All About The Human Genome Project
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Milestones in Genetics: TimelineAn interactive timeline of milestones in genetics, presenting more than 90 key events and discoveries. By clicking on any year in the timeline, the user can read a short, illustrated story and often find archival images and original scientific publications.
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Instructor: |
Dr. Yu-Wai Peter Lin Office: SNHS 330
Phone: (305)899-3226
Office Hours: |
Dr Lin: Mon and Wed 9:00 AM - 11:30 AM
Posted on office door or by appointment
Genetics Lab Class Distribution List (Bucmail): |
Lab 1 : BIO-341L-L1-0501@mail.barry.edu
How do I send email directly without searching the global address book ?
The class list addresses are created using the following format:
Course-ID-Section-YearTerm@mail.barry.edu
For example, Accounting 336 01 held in Fall 2001 would have ACC-336-01-0101@mail.barry.edu as the e-mail address.
Will everyone be able to send messages to my class list ?
No, only members (students registered in the course) of the list will be able to send to the class list. Instructors are added as members to the class list, allowing them to send messages.
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Barry's official means of communication with students is BUCMAIL. A student email account is automatically created by the Division of Information Technology for all registered students. The University will communicate with students through this official address, which is recorded in the University's electronic directories and records for each student. All students taking classes that meet on the Barry University campus are required to log in to their official Barry University student email account within one week of the beginning of the semester when they enroll. Faculty or technology coordinators associated with distance education classes or courses that meet off campus should also require students to login to their official Barry University student email accounts
The following are important points to remember about student use of Barry email as well as faculty responsibilities.
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Students should check
their email as often as |
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Students must maintain
their mailboxes to |
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The practice of having
Barry email forwarded |
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In general, email is not
an appropriate medium
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Genetics Lab Objectives | |||
Lab Schedule : |
| Links and Assignments |
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Major Report (150 total possible points)
Title Page: Include the title of the experiment along with your name, the date and the course title.
Introduction: (New Page) In this section you should give a clear, detailed background of the subject in question. While the hand-out has some background, it is usually no where near being complete. You should utilize other sources. This may include your textbook, review articles, and original research journal articles on the subject. The acceptable format for referencing the cited literature in the body of the text and in the reference section is given below. After your discussion of the background and your clear identification of the subject, clearly state your hypothesis or define the problem with which the laboratory exercise deals. Relate the problem addressed by the laboratory exercise to pertinent observations or information already known from the literature. (50 points possible)
Materials and Methods: Describe the materials and methods in enough detail that the work could be repeated by others. Do not go into unnecessary detail. The style of this section should resemble that in the Proceedings of the National Academy of Sciences (PNAS). Therefore, this section should not include simply a list of the materials used or resemble a recipe book as your lab manual does. Rather the methods should be in concise sentences and paragraphs making special note of how the protocol utilized deviated from that in the laboratory manual. Also, well known methods used without significant modification need not be described but merely cited. For example, "The amount of protein was determined by the method of Bradford (1976) using bovine serum albumin as the standard." (20 points possible)
Results: The data should be presented in a clear manner by the use of properly labelled graphs and tables. Again, consult the Proceedings of the National Academy of Sciences for their style of presentation. The figures should have legends containing sufficient information that they can stand alone. Raw data used to generate the graphs should usually not be included. Although at times the raw material, once properly labelled, is your figure. For example, the picture of the DNA gel that shows the fragment sizes generated by the restriction enzymes, or the copy of the SDS PAGE gel that shows the molecular weight of proteins are both acceptable figures. Make sure to assign numbers to your tables (e.g. Table 1) and figures (e.g. Figure 5) so that you can call attention to important parts of the data in the text portion of the Results or Discussion section. (30 points possible)
Discussion: The purpose of this section is to discuss the meaning or significance of the results especially in light of previous studies. Therefore the literature you used in the introduction is again going to be important here to discuss how your results compare or add new insight to that previously described. It is in this section that you can interpret and speculate on the meaning of your results, especially based on the previous studies. Do your results corroborate or go against previous results? Using your background materials as a basis for argument, what do your results mean? Again be sure to properly cite references utilized using the format shown below. (45 points possible)
References: This section is an alphabetical list of all reference materials that have been cited in the report. Use the style listed below for citation in the text and in the reference section. (5 points possible)
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Examples of References and
Appropriate Methods of Citation
A. Within the text
In the body of the paper, citation to information from other sources are cited in this manner:
"Jones (2004) discovered that..........."
"..........was found by Jones and Edwards (2002) that......."
"High pressure may affect the brain functions responsible for higher student grades (Smith et al., 1999)."
B. Reference section
Jones, J.J. 2004. High temperatures affect student grades. J. Amer. Physiol. 101:122-144.
Jones, J.J. 1999. Temperature has no effect on student grades. Amer. Zool. 6:52-46.
Jones, J.J., and S. Edwards. 2003. High pressure affects student grades. Amer. Zool. 4:43-65.
Smith, R., J.J. Jones, and S. Edwards. 2000. High pressure affects brain function in vertebrates. Brain Research 47:1235-1257.
Zelnich, A.C. 2004. Environmental influences on brain function. Pp. 64-78 in D. Webster ed., Neural Systems. Jones Press, New York.
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| Dr. Lin's Biology Links | |
| Assignments
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http://www.nigms.nih.gov/news/science_ed/genetics/
(
PDF Version)
Gene
Gateway: Tools for exploring the
Human Genome
Population Genetics

http://www.genome.gov/glossary.cfm?key=spectral%20karyotype%20(SKY)
NCBI GENES AND DISEASE http://www.ncbi.nlm.nih.gov/disease/
Genes & Populations: HTML version or print version (PDF) (requires free Adobe Acrobat Reader to view online)
This educational brochure explains in question-and-answer format why genetics researchers sometimes study identified populations to identify links between genes and diseases. (Also available in Spanish, "Genes y Poblaciones.")
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Molecular Biology
Bacterial Transformation
http://www.biology.com/learning/molecular/concept1.html
© 1998, Peregrine Publishers, Inc., All Rights Reserved
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The Interactive Fly ©
1995, 1996
Thomas B. Brody, Ph.D.
http://flybase.bio.indiana.edu:7081/allied-data/lk/interactive-fly/aimain/1aahome.htm
A cyberspace guide to Drosophila genes and their roles in development
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http://www.exploratorium.edu/exhibits/mutant_flies/mutant_flies.html
Mutant fruit
flies
From the Exploratorium in San Francisco
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REVIEW
The Genome Sequence of Drosophila melanogaster
http://www.sciencemag.org/feature/data/genomes/landmark.shl

Coding Content of the Drosophila Genome
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Geneticists predict that computer programs that compare human DNA with mouse DNA will uncover secrets in the human genome. (Human Genome Proj./Oak Ridge Nat. Lab.)
http://www.sciencenews.org/20000429/bob2.asp
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NetWatch
(html
version)
http://www.sciencemag.org/content/current/netwatch.shtml

HemoPDB records the proteins that switch each gene on and off and the DNA sequences to which these proteins attach. Users can also find out each gene's function and the types of cells in which it is active. The scientists who assembled HemoPDB from the literature and Web sites hope it will help researchers pinpoint other genes that might be involved in blood cell development and disorders.
bioinformatics.med.ohio-state.edu/HemoPDB

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Introduction to Virtual FlyLab
To learn more about Mendel and his experiments, follow
this link to MendelWeb
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Researchers are using the zebrafish to search for a variety of genes involved in everything from obesity to bone diseases
http://www.sciencemag.org/cgi/content/full/288/5469/1160
Science Volume 288, Number 5469 Issue of 19 May 2000, pp. 1160 - 1161
©2000 by The American Association for the Advancement of Science.
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| Assignments:
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Morgan: A Genetics Tutorial
http://morgan.rutgers.edu/MorganWebFrames/htmldocs/contents.html
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Chapter 3: Animation and Tutorial
You will not be able to access the Animation and Tutorial links listed below unless your browser has either the Macromedia Flash 5 player or the Macromedia Shockwave 8 player. The Flash Animation and Tutorial will open in a secondary window which should be closed after viewing the contents. For more information on this module please reference the "Navigating the Animation and Tutorial" section located within this course.
| © Prentice-Hall,
Inc. A Division of Pearson Education. Legal Statement |
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The Biology Place: http://www.biology.com
User ID- STU/lin
Password- *********
Interactive Learning Activities (Lab Bench):
| a) | Experimenting with Mendel's Peas http://www.biology.com/learning/peas/intro.html |
b) Genetics of Organisms (Drosophila melanogaster)
[http://www.biology.com/learning/genetics/intro.html]
by Theresa Knapp Holtzclaw
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Solving Mendelian Genetics
Problems http://www.biology.com/learning/mendelian/introduction.html
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BioCoach
Mendelian Inheritance This BioCoach Activity will help
you review the basic concepts of Mendelian genetics. http://www.biology.com/learning/inheritance/intro.html
by Peter Russell, Reed
College

| Analyzing the Genetic X Files (analyzing genetic crosses) | |
| Genetic X Files Problems |
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The Institute of Genome Research
(TIGR) http://www.tigr.org/tigr_home/index.html
TIGR Web site
http://www.tigr.org/tigr_home/tdb/tdb.html
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Designing
Experiments to Solve Restriction Mapping
Problems http://www.biology.com/learning/redlab/introduction.html
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| Reading Assignments : |
Primary Source Article
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Genome Sequence
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. S. T. Cole et.al. Nature 393:537-544 [M. tuberculosis genome sequence]
Complete genome sequence of Treponema pallidum, the syphilis spirochete. Claire M. Fraser, et. al. Science 281:375-388 (1998) (http://www.sciencemag.org) [T. pallidium genome sequence]
Lin, Y.-W. P., B. A. Rupnow, D. A. Price, R. M. Greenberg, and R. A. Wallace (1992). Fundulus heteroclitus gonadotropins 3. Molecular cloning and sequencing of beta subunits of two distinct gonadotropins (GTH I and GTH II) from pituitary cDNA library using the Polymerase Chain Reaction. Mol. Cell. Endocrinol. 85: 127-139. Medline Accession No. PMID: 1526312; UI: 92405806. /Abstract
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Permission to use the Copyright DNA image granted by:
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Paul A. | paul@grserv.med.jhmi.edu | Johns Hopkins
Thiessen | http://cherubino.med.jhmi.edu/~paul | University
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Clusters of Orthologous
Groups of proteins (COGs)

Phylogenetic classification of proteins encoded in complete genomes
Malaria Triad: Genetics & Genomics
Atlas
of Genetics and
Cytogenetics
in Oncology and Haematology
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Medline Search: |
(PubMed- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi)