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Journal of Experimental Zoology
Published Online: 24 Jun 1999
Copyright © 1999 Wiley-Liss, Inc.
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Reproductive Biology
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Inhibitory action of the
gonadopeptide inhibin on amphibian (Rana pipiens)
steroidogenesis and oocyte maturation
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| Yu-Wai Peter Lin *,
Teresa Petrino, Ana Marie Landin, Sindy Franco, Isabelle
Simeus |
Biology Department, School of Natural and Health
Sciences, Barry University, Miami Shores, Florida
33161
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| email: Yu-Wai Peter Lin (plin@mail.barry.edu) |
*Correspondence to Yu-Wai
Peter Lin, Biology Department, SNHS, Barry University, 11300
Northeast Second Avenue, Miami Shores, FL 33161-6695
Funded by:
 NIH-NIGMS; Grant Number: MBRS 3 S06
GM45455-05S1
MARC; Grant Number: GM08021-14
| In view of recent reports on the production of
inhibin- and activin-like proteins in lower vertebrates
and their important role during development, we have
examined the effects of the gonadopeptide inhibin in the
process of oocyte maturation using amphibian (Rana
pipiens) fully grown preovulatory ovarian follicles
cultured in vitro. In the presence of frog pituitary
homogenate (FPH), which stimulates progesterone
(P4) levels and the subsequent germinal
vesicle breakdown (GVBD), purified porcine inhibin
(35-50 IU) inhibited both of these responses in a
dose-dependent manner. Inhibin also blocked GVBD
initiated by exogenously added P4 in intact
as well as denuded oocytes. Thus, inhibin seems to act
at the follicle (granulosa) cells because it blocked
steroidogenesis and at the oocyte because it altered the
steroid-induced oocyte maturation. The
P4-treated follicles were susceptible to the
inhibin action during the first 3 hr of steroid
stimulation, which indicates that inhibin affects some
early events during the process of GVBD. Maximum
inhibitory effect was observed when P4 and
inhibin were added simultaneously at the beginning of
the incubations. Moreover, the inhibitory effect on GVBD
caused by the gonadopeptide was dependent on the length
of exposure of the follicles to inhibin. The continuous
presence of inhibin in the culture was required to block
GVBD efficiently. Data also indicate that the inhibitory
effect of inhibin was reversible. Taken together,
results from this study present evidence that inhibin
may be a relevant paracrine/autocrine regulator of
ovarian functions. J. Exp. Zool. 284:232-240,
1999. © 1999 Wiley-Liss, Inc. |
Received: 24 June 1998; Accepted: 12 November 1998
10.1002/(SICI)1097-010X(19990701)284:2<232::AID-JEZ13>3.0.CO;2-Z About DOI |
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