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BIO 295 (13) Research SSRP

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BIO 295(13)     Research SSRP       Course Syllabus

Spring 1999    Lecture & Lab   4:00-6:00 PM     Mon,Thur                  SNHS Bldg 202

Credit: 3

Instructor: Dr. Yu-Wai Peter Lin

Office/Lab: SNHS 330    Phone: (305)899-3226

e-mail: plin@mail.barry.edu emailed.gif (14893 bytes)

Office Hours: Posted on office door or by appointment

    Mon & Wed : 8:00 - 11:00 AM

 

 

Table of Contents

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Course Objectives

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Instructional Methods

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Class Schedule

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Links and Assignments

 

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Course Objectives

 

To provide insight and hands-on experience on current topics in Biomedical and Clinical Research. Our motto: "Learn Science by Doing Science". At the end of this course, students will be able: 1) to obtain first hand experience with some of the cellular and biochemical principles concerning biotechnology. 2) to become familiar with some of the equipment and techniques commonly used in recombinant DNA research. 3) to develop laboratory and library skills used in conducting experiments and writing scientific reports.

 

 

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Instructional Methods:

The lecture portion of the course will consist of oral presentations given by the instructor and supplemented with overhead transparencies and AV slides. Laboratory exercises and demonstration of modern research techniques will be included. Peer review, cooperative learning, and active discussions with the students will be encouraged. Laboratory progress reports, and homework will be assigned.

 

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Text:

No required text. I will give out photo-copies of Laboratory Protocols and necessary background information.

 

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BIOLOGY GRADING SCALE

A = 90-100%

B = 80-89%

C = 70-79%

D = 60-69%

F = 0-59%

Your Final Grade for this course will be determined as follows:

Three Lab Progress Reports (3 x 200 pt. @) = 600 total points

Quiz/homework = 300 points

Subjective: Class participation, attendance, etc. = 100 points

Total = 1000 total points possible

Final Grade = Total points earned / 1000 x 100%

Note: All exams, quizzes and written assignments are the property of the School of Natural and Health Sciences, and will be retained by the instructor after reviewing the results with the students.

 

 

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Disability Statement:

Students with documented special learning needs may want to inform the instructor so that accommodations may be made, or contact the Barry Office of Services for Students with Disabilities 305-899-3489.

 

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Dishonesty Policy:

Students should be aware that cheating will not be tolerated. Any student caught giving or receiving assistance during quizzes or using cheat sheet, etc. will receive a grade of ZERO for that quiz. This zero grade may not be dropped or made up; and WILL be used when determining the student's final grade. Any student caught cheating a second time will receive a grade of F for the course and will be referred to the program directors for disciplinary action. A plagiarized written assignment will receive a grade of ZERO. (For further information concerning the dishonesty policy, please refer to your Barry University 1998-1999 Catalog; although students are at the high school level, students must abide by the university's dishonesty policy)

 

 

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Laboratory Report and Quiz/Homework Assignments:

There will be three laboratory progress reports (worth 200 points each) and one (1) or two (2) Surprise Quiz/assignments (worth 200 points or 100 points each) during the course. Graded assignments will be returned for review within two weeks. Any assignment turned in late will be worth a maximum of 1/2 credit.

 

 

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Class Attendance:

You are expected to attend all classes. The student who is consistently late or absent will not have the same opportunities to ask questions as punctual students who attend each lecture and laboratory session. Therefore, consistent tardiness and/or consistent absence will result in a significantly lower evaluation on class participation. Daily sign-in sheets will be provided for the student's protection. A record of students who arrive late for lecture will be kept. You are responsible for all material covered in lectures or labs.

ABSENCE FROM CLASS IS NO EXCUSE FOR

MISUNDERSTANDINGS ABOUT ASSIGNMENTS OR QUIZZES.

 

 

 

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BIO 295(13) RESEARCH SSRP

Spring 1999

Tentative Class Schedule:

 

 

Jan 19 Meeting with parents

 

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First Group (Jan 21- Mar 11)

 

Session 1:

Jan 21     Isolation of DNA (lab experimentation). REPORT.

 

Session 2:

Jan 25, 28     Restriction Endonuclease-gel electrophoresis (lecture and lab.                              experimentation).

 

Session 3:

Feb 1, 4          Restriction Endonuclease-gel electrophoresis (Cont.). REPORT

 

Session 4:

Feb 8, 11          Standard Curve and Restriction Map Construction.

 

Session 5:

Feb 15, 18     DNA Fingerprinting (lecture and lab. experimentation). REPORT

 

Session 6:

Feb 22, 25     DNA Fingerprinting (Cont.).

 

Mar 1, 4         No Lab (Spring Break)

 

Mar 8, 11     Data Analysis and presentation

 

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Second Group (Mar 15 - May 6)

  

Session 1:

Mar 15     Isolation of DNA (lab experimentation).REPORT.

Mar 18     Restriction Endonuclease-gel electrophoresis   (lecture and lab. experimentation).

 

Session 2:

Mar. 22,25     Restriction Endonuclease-gel electrophoresis (Cont.). REPORT

                

Session 3:

Mar 29,     Restriction Endonuclease-gel electrophoresis (Cont.).

 

Apr 1         No Lab (Easter Break)

 

Session 4:

Apr 5, 8     Standard Curve and Restriction Map Construction

 

Session 5:

Apr 12, 15     DNA Fingerprinting (lecture and lab. experimentation). REPORT

 

Session 6:

Apr 19, 22     DNA Fingerprinting (Cont.).

 

Session 7:

Apr 26, 29     DNA Fingerprinting (Cont.).

 

Session 8:

May 3         Data Analysis

 

May 6    Presentations for Parents

 

 

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Links and Assignments

 

bulletDr. Lin's Biology Links (unframed)
bulletDr. Lin's Biology Links (framed version)
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bulletAssignments

 

CELL

Dictionary of Cell Biology
Cell and Molecular Biology Online
Biochemistry, Biophysics, and Molecular Biology
The Biology Project: Cell Biology
The Biology Project: Forensic DNA
The Biology Project: Molecular Biology
cells - homepage
CELLS alive!
Virtual Cell
Eukaryotic Animal Cell - TEM
Eukaryotic Plant Cell - TEM

Medline, NIH, CDC, GOV.

World Health Organization

 

CDC

Ctr Disease Control Prevention CDC
Morbidity & Mortality Weekly Report

 

National Library of Medicine

Internet Grateful Med - Medline
Welcome to PubMed-Nat Lib Med
U.S. National Library of Medicine (NLM)

 

 

 

bulletAssignments:

biologyplace.gif (1600 bytes)

The Biology Place: http://www.biology.com

User ID- STU/lin

Password- *********

Interactive Learning Activities:

bulletExploring Size and Scale http://www.biology.com/learning/sizeandscale/introduction.html

 

bulletDNA Replication
    DNA Structure & Replication

http://www.biology.com/learning/dnarep/intro.html

Tutorial on DNA Replication;

Leading and lagging strand ;

DNA: Meselson and Stahl's Experiment

 

bulletRestriction Endonuclease- DNA Electrophoresis; http://www.biology.com/learning/molecular/concept2.html

   

 

bio_icon_small1(1).gif (981 bytes) BioCoach

Restriction Enzyme Digestion of DNA
by Peter Russell, Reed College
© 1997, Peregrine Publishers, Inc., All Rights Reserved http://www.biology.com/learning/red/introduction.html

 

A Hypothetical (Tutorial) DNA Mapping Example http://www.biology.com/learning/red/mapping.html


 

 

DNA Fingerprint

Visit Cold Spring Harbor Laboratory's DNA Learning Center and see how science works at the molecular level with an interactive DNA fingerprint. (To view this application on your browser, you must use the Shockwave Plug-in.)

 

 

 

    

DNA Detective

http://vector.cshl.org/resources/dnadetective.html

 

DNA Fingerprinting

DNA Fingerprints, RFLPs, Slot Blots -- these are all variants of the poweful new technology that has revolutionized forensic science: DNA testing. From identifying the remains of American soldiers, to deciding paternity cases, to elminating -- and often convicting -- criminal suspects, DNA testing has become a powerful new weapon in the lawyer's arsenal.

First developed in England in 1985, DNA testing takes advantage of the fact that, with the exception of identical twins, the genetic material -- DNA -- of each person is unique. When the National Research Council said in a 1992 study that DNA testing was a reliable method to identify criminal suspects, the technology rapidly entered the mainstream court system. Today, it is hard to pick up a daily paper and not find an article reporting on the use of DNA testing in a civil or criminal court case.

The two animated tutorials below review the science and applications of DNA testing. To view each, simply click on the tutorial's link, and a new window will open on your screen. When you are finished with the tutorial, close your window and return here, where you can access the additional Internet resources listed below.

Join the discussion on DNA Fingerprinting on Cold Spring Harbor Laboratory DNA Learning Center  Message boards.

Animations

Note: you must have the Shockwave Plug-in to view this page's animations in your web browser.

DNA Fingerprinting via Southern Blotting PLAY
DNA Detective PLAY

Additional DNA Testing Internet Resources

Please note that these links are not part of the DNA Learning Center, and Cold Spring Harbor Laboratory has no control over their content or availability.

DNA Goes to Court, by Lynn Elwell, PhD.
An article on the use of DNA Testing in the courts, published in Carolina Biological Supply Company's Carolina Tips magazine, October, 1995.
DNA Fingerprinting (Microsoft Encarta Online entry)
A concise definition of DNA Fingerprinting from the Microsoft Encarta Online encyclopedia.
How scientists and forensic experts use technology to analyze evidence from crime scenes
From the University of Wisconsin’s Why Files, an article about forensic science, and why DNA testing has become the main tool of lawyers and entomologists alike.
Use of DNA in Identification
Included in the MIT Biology Hypertextbook, an excerpt from a talk given by noted DNA researcher Dr. Eric S. Lander at the "Winding Your Way through DNA" symposium, which took place at the University of California San Francisco in 1992.
An Interview with DNA Forensics Authority Dr. Bruce Weir
Part of the "About Biotech" series of articles sponsored by Genentech's Access Excellence program.


 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  LabBench

Molecular Biology
by Theresa Knapp Holtzclaw
© 1998, Peregrine Publishers, Inc. All Rights Reserved.
Development Team

Bacterial Transformation

http://www.biology.com/learning/molecular/concept1.html

   

© 1998, Peregrine Publishers, Inc., All Rights Reserved

 

 

 

 

 

 

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Interactive Study Guide --

From Gene to Protein: Transcription (By Peter Russell, Reed College)

http://www.biology.com/learning/transcription/intro.html

Ribosome Song-- www.biology.com/learning/translation/ribosome/ribosome.html

 

From Gene to Protein: Translation (Protein Synthesis)

http://www.biology.com/learning/translation/intro.html

 

 

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