(3' end) COOH-Box

 CLUSTAL W (1.82) Multiple Sequence Alignments



Sequence format is Pearson
Sequence 1: ZebrafishPc1      483 bp
Sequence 2: ZebrafishPc2      454 bp
Sequence 3: ZebrafishPc3      469 bp
Start of Pairwise alignments
Aligning...
Sequences (1:2) Aligned. Score:  6
Sequences (1:3) Aligned. Score:  10
Sequences (2:3) Aligned. Score:  8
Guide tree        file created:   [/ebi/extserv/old-work/677228.508677.dnd]
Start of Multiple Alignment
There are 2 groups
Aligning...
Group 1:                     Delayed
Group 2:                     Delayed
Sequence:3     Score:4646
Sequence:2     Score:4072
Alignment Score 2682
CLUSTAL-Alignment file created  [/ebi/extserv/old-work/677228.508677.aln]

Clustal_Alignment_Zebrafish_Polycomb1_2_3_Nucleotides

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CLUSTAL W (1.82) multiple sequence alignment

      @ D  G  E  T : Tanslation using ZebrafishPc2



ZebrafishPc1      -----ACTTCCCCGCATTCTGGAAGGAAAAGTTCTGGATTTAACACAAGTTCTGCACCAA 55
ZebrafishPc3      ---------CATTAAAAGAAAGGCAGATGAATCGTTATCTTACCGCCCTTCCAAAACCGA 51
ZebrafishPc2      CTGATGGTGAAACACCAGATCAAGGAGAAGGCCGTGAGAATGGTTCTATTGAGAAACCCA 60
                                                    *     *    *   *     *** *
                  @ D  G  E  T  P  D  Q  G  E  G  R  E  N  G  S  I  E  K  P  S

ZebrafishPc1      ACACTCCTAGCAAGTTCCAGACC-TCACAGCAAGCCTTAAAGAGTCCACAGAAATTGAAA 114
ZebrafishPc3      GAGATCAGGAGAGACCTCTAACTGTGATATCATGCATT-----TTACCCAGAAATACAAA 106
ZebrafishPc2      GTGAATATGTTCGCCAGCGAAGGTAGACGCCAAATCAA----ATCACCTTAAGAGGCATC 116
                                   *  *     *   **              *    * *   *  

ZebrafishPc1      GCGGATGATTTGGCTGAAAGACTTGGGAAGAAAAGCCAAGCTAGAACAG-AAAAGATTCT 173
ZebrafishPc3      GCAGAAACCACCCATGATGGGAAGCAAATGGGAAGCAGATCCAGTGATG-TGAAATTTTC 165
ZebrafishPc2      TTTCAGAGCCCAGCGAGGACGTGAGAAACTGTAAGCAGCTCCTGAGCTTCAGGAGCATCA 176
                      *                      *    ****    *  *         *   *  

ZebrafishPc1      GC-CAACAGAGGGTCG--AGATTCTCAACCGGCGCAAGACAGACCCTCGTCTAAAGATCC 230
ZebrafishPc3      ACATGGTGGAAGCTTCTTAAAAGCTGGGCTAG-GCATTTTGGGACATCGACAAAAGGTTG 224
ZebrafishPc2      GTGCGCCAAACTCTTC-----CCCTCAGAGAGAAC-CTATAAACCTGCATTACAAAAGCA 230
                           *   *         **      *  *         *  *     **     

ZebrafishPc1      CAGCAA-ACAGAGCAAGACCTTGAGCGAATTGAGCACCGGAGAGGAGGGAAGCAGCTCCG 289
ZebrafishPc3      GCTCTG-GTGGTGCCATAAACC-AACAAGCTAAGATGAAGCATCCTCCCAAAAACAATCT 282
ZebrafishPc2      GCCCTGTGGATAATTACGACTTTAGCGACTCTATTCCCGAAGAGCCTATTGATTTGAGCG 290
                     *           *       * * *    *                         * 
                                                                 @  D  L  S  A

ZebrafishPc1      ATACCGACCATGATTCCTCATTTCCCAGGGACAGCCATGACTTGTCCATCTCTGTGCAAG 349
ZebrafishPc3      ATATCGATCC--ACTGATCAAA-CCAGAGAGCAGCTCTCTCTGAGCTTTGTAGATG-ATA 338
ZebrafishPc2      CCAAAACTCTAAAACGGTTCACTGCTTTGGACAA---AGTTTCAGGAAGCTCAGCACAAA 347
                    *     *   *    *      *   *  **        *        *      *  
                    K  T  L  K  R  F  T  A  L  D  K     V  S  G  S  S  A  Q  M

                                                              COOH-Box

                                                           #   F  V  T  D  V
ZebrafishPc1      CAGGTCAGGATTGGAGGCCGACACGGAGCCTG-ATTGAGCATGTGTTTGTCACTGACGTC 408
ZebrafishPc3      CAGACCAGTCCTGGCTGCCTTGTTTGAAGAAT-ATGGAGAAAATTGTTGTTACTGATGTA 397
ZebrafishPc2      TGGA-CAAAACAGACAGCCAATGTAAAGCCTTCGTGGGAAACGTTATCATCACTGATATC 406
                    *  **     *   ***       *       * *   *  *  *  * *****  * 
                    D   K  T  D  S  Q  C  K  A  F  V  G  N  V  I  I  T  D  I

                  T  A  N  L  V  T  V  T  V  K  E  S  P  T  S  V  G  F  F  S
ZebrafishPc1      ACTGCAAACCTGGTCACAGTAACAGTGAAGGAATCTCCAACCAGCGTTGGGTTTTTTAGC 468
ZebrafishPc3      ACAAGTAACTCATTGACTGTAACCATAAAAGAGAGTTCAACAGACAAAGGGTTCTTTAAA 457
ZebrafishPc2      ACCACGAACTGCTTGACCGTTACCTTTAAAGAA--TACGTTCAAGAATGA---------- 454
                  **    ***    * ** ** **  * ** **   * *          *           
              @   T  T  N  C  L  T  V  T  F  K  E    Y  V  Q  E  X

                  I  R  N  Y  X
ZebrafishPc1      ATTCGGAATTATTGA 483
ZebrafishPc3      GAAAACAGATAA--- 469
ZebrafishPc2      ---------------
                                 

(
ZebrafishPc1:0.45664,
ZebrafishPc2:0.47948,
ZebrafishPc3:0.43462);