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| Summary: |
Analysis of brains, hearts, and lungs of 9
individuals, 3 each collected from Maine, New Jersey, and Georgia.
192 metabolic genes examined. Results provide insight into the
variation in tissue-specific gene expression among outbred
individuals and among different natural populations of a
species.
Parent Platform: GPL1224
Reference
Series:
GSE2293 |
| Type: |
gene expression array-based count |
| Subsets: |
9 individual, 3 tissue sets. |
| Samples: |
108 (listing 18) |
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| Summary: |
(Submitter supplied) Examination of the expression of
a selected suite of 192 metabolic genes in brain, heart and liver in
three populations of the teleost fish Fundulus heteroclitus using a
highly replicated experimental design.
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related DataSet
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related Platform |
| Samples: |
108 (listing 18) |
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| Summary: |
(Submitter supplied) Cr(III) is the dominant toxicant
at some Superfund sites within the United States and therefore we
are interested in its effects. Cr(III)’s mechanisms are not well
studied or understood because of its low bioavailability. We have
attempted to characterize the effects of Cr(III) on gene expression
in the liver of adult male Fundulus heteroclitus. The NOEC and LOEC
were determined at 32 and 64mg/L, respectively, by measuring growth
after exposing juveniles for 30 days. Secondary exposures were
performed with adult males at 32mg/L, livers excised, and RNA
extracted. Microarrays were probed with cDNA from untreated or
Cr(III)-exposed adult fish and gene expression was quantified.
Cr(III) at 32mg/L altered the expression of 5 genes, including
GSTalpha, GSTtheta, and ALDH4. Ultimately, we anticipate using this
gene expression information to determine whether chromium is
available at potentially adverse concentrations in contaminated
sites.
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related Platform |
| Samples: |
8 |
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| Summary: |
(Submitter supplied) Microarrays were printed using
192 cDNAs from a F. heteroclitus cardiac library encoding essential
proteins for cellular metabolism. These cDNAs were a subset of over
33,000 expressed sequences in our database, which all may be found
at http://genomics.rsmas.miami.edu/funnybase/super_craw3/ (Oleksiak
et al. 2001). These 192 cDNAs were amplified with amine-linked
primers and printed on 3-D Link Activated slides (Surmodics Inc.,
Eden Prairie, MN) using a SpotArray Enterprise piezoelectric
microarray printer (PerkinElmer Life Sciences Inc., Boston, MA, USA)
at Louisiana State University. Slides were blocked following slide
manufacturer protocols. The suite of 192 amplified cDNAs was printed
as a group in spatially separated replicates (6 replicates for 178
genes, and 4 replicates for 14 genes). Four hybridization zones of
these six replicate arrays were printed per slide, with each zone
set separated by a hydrophobic barrier.
more...
1
related DataSet
1
related Series |
| Samples: |
108 (listing 18) |
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