Folder: Pc2 Analysis Nov 15 02


File: Clustalw_Alignment_Zebrafish_Polycomb2_Protein_Human_Mouse_Chicken_Frog_1

 CLUSTAL W (1.82) Multiple Sequence Alignments

Sequence format is Pearson
Sequence 1: ZebrafishPc2 Nov 22 02   477 aa
Sequence 2: HumanPc2 >gi|2317723]    558 aa
Sequence 3: MousePc2 >gi|2781392]    551 aa
Sequence 4: ChickenPc2 >gi|3649787]  523 aa
Sequence 5: XenopusPc2 >gi|1086556]  521 aa
Start of Pairwise alignments
Aligning...
Sequences (1:2) Aligned. Score:  44
Sequences (1:3) Aligned. Score:  43
Sequences (1:4) Aligned. Score:  45
Sequences (1:5) Aligned. Score:  45
Sequences (2:3) Aligned. Score:  89
Sequences (2:4) Aligned. Score:  60
Sequences (2:5) Aligned. Score:  49
Sequences (3:4) Aligned. Score:  61
Sequences (3:5) Aligned. Score:  51
Sequences (4:5) Aligned. Score:  53
Guide tree        file created:   [/ebi/extserv/old-work/471092.143963.dnd]
Start of Multiple Alignment
There are 4 groups
Aligning...
Group 1: Sequences:   2      Score:11301
Group 2: Sequences:   3      Score:8952
Group 3: Sequences:   4      Score:7958
Group 4: Sequences:   5      Score:6960
Alignment Score 15621
CLUSTAL-Alignment file created  [/ebi/extserv/old-work/471092.143963.aln]

CLUSTAL W (1.82) multiple sequence alignment


HumanPc2          MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNR 60
MousePc2          MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNR 60
ChickenPc2        MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNR 60
XenopusPc2        MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSSKYNTWEPEENILDPRLLVAFQNR 60
ZebrafishPc2      MELPAVGEHVFAVEGIEKKRLRKGRMEYLVKWRGWSPKYNTWEPEENILDPRLLVAFQNR 60
                  **************.*****:****:**********.*****************:*****

HumanPc2          ERQEQLMGYRKRGPKPKPLVVQVPTFARRSNVLTGLQDSSTD--NRAKLDLGAQGKGQGH 118
MousePc2          ERQEQLMGYRKRGPKPNPLVVQVPTFARRSNVLTGLQDSSAD--NRAKLELGTQGKGQGH 118
ChickenPc2        ERQEQLMGYRKRGPKPKPLVVQLPSFARRSNILTGLQDPTVD--TRPKLDLGSSGKSQQH 118
XenopusPc2        ERQEQIMGYRKRGPKPKNNLVSMPSFARRSNVLSGLHDSSGE--NRTKLDLGPIPKS--H 116
ZebrafishPc2      ERQEQMVGYRKRGPKPKHPLIQLPAFARRSSILGGLQDTSLDEENQPKVDSLQMHRSRPQ 120
                  *****::*********:  ::.:*:*****.:* **:*.: :  .:.*::     :.  :

HumanPc2          QYELNSKKHHQYQPHSKE--GKPRPPGKSGKYYYQLNSKKHHPYQPDPKMYDLQYQGGHK 176
MousePc2          QYELNSKKHHQYQPHSKERSGKPPPARKSGKYYYQLNSKKHHPYQPDPKMYDLQYQGGHK 178
ChickenPc2        QYELNSKKHHQYQPNGKE-SGVKHQSHSKGKYYYQLNSKKHHHYQPDPKMYEPHYHPSGK 177
XenopusPc2        QYQLNSKKHHQYQPSGKD-HNEKHHSSNKKKYYYQLNSKKHHHYQPDPKLYEQYTIEKES 175
ZebrafishPc2      HYQLNSKKHHQYQPSCKEISVEQHMSGKK-KHFYQLNSKKHHHYQPDPKMYDTPLTGP-K 178
                  :*:***********  *:       . .. *::********* ******:*:       .

HumanPc2          EAPSPT---CPDLGAKSHP---PDKWAQGAGAKGYLGAVKP--LAGAAGAPGKGSEKGPP 228
MousePc2          EAPSPT---CPDLGTKSHP---PDKWAHGAAAKGYLGAVKP--LGGGAGAPGKGSEKGPP 230
ChickenPc2        EPQGQA---CLDS-TKSPLGAHPDKWAHG-AAKNLLGPVKN--LAAES---KNGAEKNLS 227
XenopusPc2        QISTDVRNRHRDSLTHTQAADMGTQLKNG-TDSVISGPERNGICNGVSSSPSTEMSSSPS 234
ZebrafishPc2      EVKGQD----PSNKGWNLPPALQQKWIRN-KDTGCLSKVKD--LSIELKGLPDNANKAER 231
                  :          .    .       :  ..   .   .  :               ..   

HumanPc2          NGMMPAPKEAVTGNGIGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIKSGEVAEGEAR 288
MousePc2          NGMTPAPKEAVTGNGIGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIKSGEAAEGEAR 290
ChickenPc2        SGTGPPPRDRVTSNGLGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIKSGEPPR---- 283
XenopusPc2        TELSSSPSTELPSSGVGGKMKIVKNKNKNGRIVIVMSKYMENGMQSVKIKSSEEDCDMG- 293
ZebrafishPc2      T-LKTSAKEFALPNGISSKMKIIKNKNKNGRIVIVMSKYMDKGVHSSKVKN--------- 281
                  .   ...      .*:..****:*****************::*::: *:*.         

HumanPc2          SPSHKKRAADERHPP--ADRTFKKAAGA-EEKKVEAPPKRREEEVSGVSD-PQPQDAGSR 344
MousePc2          SPSHKKRAAEERHPQ--GDRTFKKAAGASEEKKAEVPCKRREEEALVSGD-AQPQDLGSR 347
ChickenPc2        -----KRAPEERTAKKGGEEKLEAWRKPGEERAVGSNGLSAAEGESRQPA-AELQ-EGPR 336
XenopusPc2        --DVRRRFDSPGTLN--GDKTCTAQEEKTEHWKKRVESRVKIHEGSKSVDKGSVHLAGLR 349
ZebrafishPc2      -----RDVSQVGTVH--NEEPPVNGTDNRSDGETPDQGEGRENGSIEKPS-EYVDEKSLR 333
                       :   .        :.         ..           .           .  . *

HumanPc2          KLSPTKEA---FGEQPLQLTTKPDLLAWDPARNTHPPSHHPHPHPHHHHHHHHHHHHAVG 401
MousePc2          KLSPTKEA---FGEQPLQLTTKPDLLAWDPARSSHPPAHH------HHHHHHHHHHHTVG 398
ChickenPc2        KPSPGKELPLPVAEQPLQLTTKPDLVPWSLSPGRE------------------HSPSSMG 378
XenopusPc2        RTYSTASET--LNDQPLQLTTKSSHVPMPNRTRSP------------------VYAGEPY 389
ZebrafishPc2      KDMP--------GQKATEICDRPNSSPAKVDAKSN------------------------- 360
                  :  .         ::. ::  :..  .                                 

HumanPc2          LNLS--HVRKRCLSETHGEREPCKKRLTARSISTP----TCLGGSPAAERPADLPP-AAA 454
MousePc2          LNLS--HARKRCLSETHGEREPCKKRLTARSISTP----TCLGGSPVSEHPANVSPTAAS 452
ChickenPc2        LNLSSPGARKRCLSEPHGDREPGKKRLTSRSISAP----TCLG----PERPE-------L 423
XenopusPc2        NDLVYTNPRKRCLSEANGNKELCKKTLTSRSVSAPGIV-ECKG----GLTPLN-----VP 439
ZebrafishPc2      -------HLKRHLSEPSEDVRNCKQLLSFRSISAPNSSP--------QREPINLHY--KS 403
                           ** ***.  : .  *: *: **:*:*               *         

HumanPc2          LRQPEVILLDSDLDEPIDLRSVKSRSEAGEPPSSLQVKPETPASAAVAVAAAAAPTTTAE 514
MousePc2          LPQPEVILLDSDLDEPIDLRCVKMRSDAGEPPSTLQVKPEAP-----AVAAVVAPAPASE 507
ChickenPc2        PAQPEVILLDSDLDEPIDLRCVKPRAEG--EAALVQVKPEEPP--APAEQPPVEPPQQPR 479
XenopusPc2        LQEPDIILLDSDLDEPIDLRCVKSRCDS--------------------DQEVDKPEIQVT 479
ZebrafishPc2      SPVDNYDFSDSIPEEPIDLS----------------------------AKTLKRFTALDK 435
                      :  : **  :*****                                         

HumanPc2          KPPAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYVTV 558
MousePc2          KPPAEAQEEPVEPLSEFKPFFGNIIITDVTANCLTVTFKEYVTV 551
ChickenPc2        QAAEEEEEEEAEGLREFKPFFGNIIITDVTANCLTVTFKEYVTV 523
XenopusPc2        QNPQSVDVDVCE--SQFKPFFGNIVITDVTANCLTVTFKEYITV 521
ZebrafishPc2      VSGSSAQMDKTD--SQCKAFVGNVIITDITTNCLTVTFKEYVQE 477
                      . : :  :   : *.*.**::***:*:**********:  

(
ZebrafishPc2:0.31118,
(
(
HumanPc2:0.05701,
MousePc2:0.04825)
:0.15152,
ChickenPc2:0.18687)
:0.04625,
XenopusPc2:0.23809);