Polycomb Pc2-like Protein Alignment

(Human, Mouse, Chicken and frog)

Click here to view Protein Alignment

Your ClustalW Results:

Pairwise Scores:

 CLUSTAL W (1.81) Multiple Sequence Alignments



Sequence format is Pearson

Sequence 1: Human gi|2317723|gb|AAB80718.1| Polycomb 2 homolog [Homo sapiens]    558 aa

Sequence 2: Mouse gi|2781392|gb|AAB96874.1| transcriptional repressor Mpc2 [Mus musculus]  551 aa

Sequence 3: Chicken gi|3649787|dbj|BAA33402.1| chromobox protein (CHCB3) [Gallus gallus]   523 aa

Sequence 4: Frog gi|1086556|gb|AAC59728.1| XPolycomb [Xenopus laevis]         521 aa

Start of Pairwise alignments
Aligning...
Sequences (2:3) Aligned. Score:  61
Sequences (1:2) Aligned. Score:  89
Sequences (3:4) Aligned. Score:  53
Sequences (2:4) Aligned. Score:  51
Sequences (1:3) Aligned. Score:  60
Sequences (1:4) Aligned. Score:  49
Guide tree        file created:   [/net/nfs0/vol1/production/w3nobody/tmp/644929.598893-161944.dnd]
Start of Multiple Alignment
There are 3 groups
Aligning...
Group 1: Sequences:   2      Score:11301
Group 2: Sequences:   3      Score:8952
Group 3: Sequences:   4      Score:7947
Alignment Score 11215
CLUSTAL-Alignment file created  [/net/nfs0/vol1/production/w3nobody/tmp/644929.598893-161944.aln]

Your Multiple Sequence Alignment:

Polycomb Pc2-like Protein Alignment

CLUSTAL W (1.81) multiple sequence alignment

              Forward Primer                  
Human           MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNR 60
Mouse           MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNR 60
Chicken         MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNR 60
Frog            MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSSKYNTWEPEENILDPRLLVAFQNR 60
                ************************************.*****************:*****

Human           ERQEQLMGYRKRGPKPKPLVVQVPTFARRSNVLTGLQDSSTDNRAKLDLGAQGKGQGHQY 120
Mouse           ERQEQLMGYRKRGPKPNPLVVQVPTFARRSNVLTGLQDSSADNRAKLELGTQGKGQGHQY 120
Chicken         ERQEQLMGYRKRGPKPKPLVVQLPSFARRSNILTGLQDPTVDTRPKLDLGSSGKSQQHQY 120
Frog            ERQEQIMGYRKRGPKPKNNLVSMPSFARRSNVLSGLHDSSGENRTKLDLGPIPKS--HQY 118
                *****:**********:  :*.:*:******:*:**:*.: :.*.**:**.  *.  ***

                    Reverse Primer 
Human           ELNSKKHHQYQPHSKE--GKPRPPGKSGKYYYQLNSKKHHPYQPDPKMYDLQYQGGHKEA 178
Mouse           ELNSKKHHQYQPHSKERSGKPPPARKSGKYYYQLNSKKHHPYQPDPKMYDLQYQGGHKEA 180
Chicken         ELNSKKHHQYQPNGKE-SGVKHQSHSKGKYYYQLNSKKHHHYQPDPKMYEPHYHPSGKEP 179
Frog            QLNSKKHHQYQPSGKD-HNEKHHSSNKKKYYYQLNSKKHHHYQPDPKLYEQYTIEKESQI 177
                :*********** .*:  .    . .. ************ ******:*:       .: 

Human           PSPT---CPDLGAKSHP---PDKWAQGAGAKGYLGAVKP--LAGAAGAPGKGSEKGPPNG 230
Mouse           PSPT---CPDLGTKSHP---PDKWAHGAAAKGYLGAVKP--LGGGAGAPGKGSEKGPPNG 232
Chicken         QGQA---CLDS-TKSPLGAHPDKWAHGA-AKNLLGPVKN--LAAES---KNGAEKNLSSG 229
Frog            STDVRNRHRDSLTHTQAADMGTQLKNGT-DSVISGPERNGICNGVSSSPSTEMSSSPSTE 236
                   .     *  :::       :  :*:  .   *. :     . :    .  ... .. 

Human           MMPAPKEAVTGNGIGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIKSGEV----AEGE 286
Mouse           MTPAPKEAVTGNGIGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIKSGEA----AEGE 288
Chicken         TGPPPRDRVTSNGLGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIKSGEP----PRKR 285
Frog            LSSSPSTELPSSGVGGKMKIVKNKNKNGRIVIVMSKYMENGMQSVKIKSSEEDCDMGDVR 296
                  ..*   :...*:*****************************:*****.*        .

Human           ARSPSHKKRAADERHPPADRTFKKAAGA-EEKKVEAPPKRREEEVSGVSDPQPQDAGSRK 345
Mouse           ARSPSHKKRAAEERHPQGDRTFKKAAGASEEKKAEVPCKRREEEALVSGDAQPQDLGSRK 348
Chicken         APEERTAKKGGEEKLEAWRK-------PGEERAVGSNGLSAAEGESRQPAAELQ-EGPRK 337
Frog            RRFDSPGTLNGDKTCTAQEEK-----TEHWKKRVESRVKIHEGSKSVDKGSVHLAGLRRT 351
                       .  .::      .          :: .                .       *.

Human           LSPTKEA---FGEQPLQLTTKPDLLAWDPARNTHPPSHHPHPHPHHHHHHHHHHHHAVGL 402
Mouse           LSPTKEA---FGEQPLQLTTKPDLLAWDPARSSHPPAHH------HHHHHHHHHHHTVGL 399
Chicken         PSPGKELPLPVAEQPLQLTTKPDLVPWSLSPGRE------------------HSPSSMGL 379
Frog            YSTASET---LNDQPLQLTTKSSHVPMPNRTRSP------------------VYAGEPYN 390
                 *. .*    . :********.. :.                                  

Human           NLS--HVRKRCLSETHGEREPCKKRLTARSISTP---TCLGGSPAAERPADLPP-AAALR 456
Mouse           NLS--HARKRCLSETHGEREPCKKRLTARSISTP---TCLGGSPVSEHPANVSPTAASLP 454
Chicken         NLSSPGARKRCLSEPHGDREPGKKRLTSRSISAP---TCLG----PERPE-------LPA 425
Frog            DLVYTNPRKRCLSEANGNKELCKKTLTSRSVSAPGIVECKG----GLTPLN-----VPLQ 441
                :*     *******.:*::*  ** **:**:*:*    * *       *           

Human           QPEVILLDSDLDEPIDLRSVKSRSEAGEPPSSLQVKPETPASAAVAVAAAAAPTTTAEKP 516
Mouse           QPEVILLDSDLDEPIDLRCVKMRSDAGEPPSTLQVKPEAP-----AVAAVVAPAPASEKP 509
Chicken         QPEVILLDSDLDEPIDLRCVKPRAEG--EAALVQVKPEEPP--APAEQPPVEPPQQPRQA 481
Frog            EPDIILLDSDLDEPIDLRCVKSRCDS--------------------DQEVDKPEIQVTQN 481
                :*::**************.** *.:.                          *     : 

Human           PAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYVTV 558
Mouse           PAEAQEEPVEPLSEFKPFFGNIIITDVTANCLTVTFKEYVTV 551
Chicken         AEEEEEEEAEGLREFKPFFGNIIITDVTANCLTVTFKEYVTV 523
Frog            PQSVDVDVCE--SQFKPFFGNIVITDVTANCLTVTFKEYITV 521
                . . : :  *   :********:****************:**

Your guide tree:

(
(
Human:0.06078,
Mouse:0.04449)
:0.15439,
Chicken:0.18399,
Frog:0.28434);