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BIO 330 (02) CELL BIOLOGY

CONCEPTS & ASSIGNMENT

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bulletAssignments:

Contents

bulletThe Biology Place
bulletCentral Dogma of Molecular Biology
bulletMembrane Structure and Transport
bulletMembrane Dynamic and Communication
bulletGlycolysis and Cellular Respiration
bulletPhotosynthesis
bullet DNA: Instruction Manual for all Life
bulletDNA Structrue and Replication
bulletThe Lac Operon in E. coli
bulletElectrophoresis- Restriction Enzyme
bulletDNA Learning Center- Cold Spring Harbor Lab.(CSHL)
bulletHow DNA is Sequenced: Sanger 
bulletDNA Fingerprinting
bulletPolymerase Chain Reaction (PCR)
bulletDNA Sequencing
bulletTranscription and Translation
bulletBacterial Transformation
bulletInvestigating the Bacterial Genome
bulletThe Institute for Genomic Research (TIGR)
bulletWebQuest
bulletGene Gateway: Tools for exploring the Human Genome

 

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biologyplace.gif (1600 bytes)© 1999, Peregrine Publishers, Inc., All Rights Reserved  

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The Biology Place: http://www.biology.com

User ID- STU/lin

Password- *********

Interactive Learning Activities:

bulletExploring Size and Scale http://www.biology.com/learning/sizeandscale/introduction.html

            by Thomas Terry, University of Connecticut
                © 1996, Peregrine Publishers, Inc., All Rights Reserved

1 Angstrom Size

[ruler]

hydrogen atom 

Model of a hydrogen atom.
(© Tom Terry)

 

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bulletThe central dogma of molecular biology describes the two-step process, transcription and translation, by which the information in genes flows into proteins: DNA RNA protein.
bullethttp://www.biology.com/learning/transcription/overview.html

© 1998, Peregrine Publishers, Inc., All Rights Reserved

 

The MIT Biology Hypertextbook

The Experimental Study Group
Massachusetts Institute of Technology
Copyright 1996, 1997, 1998, Massachusetts Institute of Technology

http://esg-www.mit.edu:8001/bio/7001main.html

  Central Dogma Directory ( MIT HyperTextbook)

http://esg-www.mit.edu:8001/bio/dogma/dogmadirnew.html

 

 

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Biomembranes I:
Membrane Structure & Transport

by Tom Terry, University of Connecticut
© 1999, Peregrine Publishers, Inc. All Rights Reserved.
Development Team

This BioCoach activity is designed to help students review the structure of membranes and the variety of ways in which materials travel across membranes by active or passive processes

http://www.biology.com/learning/biomembrane1/intro.html

 

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Biomembranes II:
Membrane Dynamics and Communication

by Tom Terry, University of Connecticut
© 1999, Peregrine Publishers, Inc. All Rights Reserved.
Development Team
 

http://www.biology.com/learning/biomembrane2/intro.html

In this BioCoach activity, students can review how regions of membrane pinch inward or bud outward to allow materials to move through cells; how membranes mediate the communication of signals; and how specialized membrane regions bind different cells into tissues.

 

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Cell Respiration
by Graham Kent, Smith College
© 1999, Peregrine Publishers, Inc. All Rights Reserved.
Development Team

ATP process

http://www.biology.com/learning/cellresp/intro.html

 

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Photosynthesis
by Graham Kent, Smith College
© 1997, Peregrine Publishers, Inc., All Rights Reserved

http://www.biology.com/learning/photosynth/introduction.html

 

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bulletDNA Replication
    DNA Structure & Replication

http://www.biology.com/learning/dnarep/intro.html

DNA Structure and Replication
by Peter Russell, Reed College
© 1998, Peregrine Publishers, Inc. All Rights Reserved.
Development Team

 

 

 

Tutorial on DNA Replication;

http://www.biology.com/news/cells/replicat.html

© 1998, Peregrine Publishers, Inc., All Rights Reserved

 

Leading and lagging strand ; http://www.biology.com/kimball/D/DNAReplication.html

 

© 1998, Peregrine Publishers, Inc., All Rights Reserved

 

           

 

 

 

 

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DNA: Meselson and Stahl's Experiment http://www.biology.com/learning/dnarep/experiment.html

 

© 1998, Peregrine Publishers, Inc., All Rights Reserved

 

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bulletThe lac Operon in E. coli
by Deanna Raineri, University of Illinois at Urbana-Champaign
© 1998, Peregrine Publishers, Inc. All Rights Reserved.
Development Team

http://www.biology.com/learning/lacoperon/intro.html

Copyright 1998, Peregrine Publishers, Inc. All Rights Reserved.

 

 

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bulletRestriction Endonuclease- DNA Electrophoresis; http://www.biology.com/learning/molecular/concept2.html

   

© 1997, Peregrine Publishers, Inc., All Rights Reserved

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bio_icon_small1(1).gif (981 bytes) BioCoach

Restriction Enzyme Digestion of DNA
by Peter Russell, Reed College
© 1997, Peregrine Publishers, Inc., All Rights Reserved http://www.biology.com/learning/red/introduction.html

 

A Hypothetical (Tutorial) DNA Mapping Example http://www.biology.com/learning/red/mapping.html


© 1997, Peregrine Publishers, Inc., All Rights Reserved

 

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http://vector.cshl.org/index1.html

All Rights Reserved 

DNA From the Beginning   http://vector.cshl.org/dnaftb/    © 1998 Cold Spring Harbor Laboratory

 

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    © 1998 Cold Spring Harbor Laboratory

How DNA is sequenced : Sanger Chain Termination Sequencing

http://vector.cshl.org/dnaftb/23/animation/

 

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DNA Fingerprint

Visit Cold Spring Harbor Laboratory's DNA Learning Center and see how science works at the molecular level with an interactive DNA fingerprint. (To view this application on your browser, you must use the Shockwave Plug-in.) http://vector.cshl.org/resources/resources.html

 

  All Rights Reserved

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http://vector.cshl.org/index1.html

DNA Detective All Rights Reserved

http://vector.cshl.org/resources/dnadetective.html

 

DNA Fingerprinting

DNA Fingerprints, RFLPs, Slot Blots -- these are all variants of the poweful new technology that has revolutionized forensic science: DNA testing. From identifying the remains of American soldiers, to deciding paternity cases, to elminating -- and often convicting -- criminal suspects, DNA testing has become a powerful new weapon in the lawyer's arsenal.

First developed in England in 1985, DNA testing takes advantage of the fact that, with the exception of identical twins, the genetic material -- DNA -- of each person is unique. When the National Research Council said in a 1992 study that DNA testing was a reliable method to identify criminal suspects, the technology rapidly entered the mainstream court system. Today, it is hard to pick up a daily paper and not find an article reporting on the use of DNA testing in a civil or criminal court case.

The two animated tutorials below review the science and applications of DNA testing. To view each, simply click on the tutorial's link, and a new window will open on your screen. When you are finished with the tutorial, close your window and return here, where you can access the additional Internet resources listed below.

Join the discussion on DNA Fingerprinting on Cold Spring Harbor Laboratory DNA Learning Center  Message boards.

Animations

Note: you must have the Shockwave Plug-in to view this page's animations in your web browser.

DNA Fingerprinting via Southern Blotting PLAY

DNA Detective

PLAY

Additional DNA Testing Internet Resources

Please note that these links are not part of the DNA Learning Center, and Cold Spring Harbor Laboratory has no control over their content or availability.

DNA Goes to Court, by Lynn Elwell, PhD.
An article on the use of DNA Testing in the courts, published in Carolina Biological Supply Company's Carolina Tips magazine, October, 1995.
DNA Fingerprinting (Microsoft Encarta Online entry)
A concise definition of DNA Fingerprinting from the Microsoft Encarta Online encyclopedia.
How scientists and forensic experts use technology to analyze evidence from crime scenes
From the University of Wisconsin’s Why Files, an article about forensic science, and why DNA testing has become the main tool of lawyers and entomologists alike.
Use of DNA in Identification
Included in the MIT Biology Hypertextbook, an excerpt from a talk given by noted DNA researcher Dr. Eric S. Lander at the "Winding Your Way through DNA" symposium, which took place at the University of California San Francisco in 1992.
An Interview with DNA Forensics Authority Dr. Bruce Weir
Part of the "About Biotech" series of articles sponsored by Genentech's Access Excellence program.


All Rights Reserved  © 1997 Cold Spring Harbor Laboratory

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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bulletPolymerase Chain Reaction (PCR)

©   Copyright 1998 Genetech, Inc.  All rights reserved

 

Biology Animation Library

http://vector.cshl.org/Shockwave/pcranwhole.html

Polymerase Chain Reaction

PLAY

© 1994 Cold Spring Harbor Laboratory

 

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bulletDNA Sequencing

Look up DNA Sequencing in the NHGRI Talking Glossary

 [-- DNA sequencing --]

defined: Determining the exact order of the base pairs in a segment of DNA.

explained: Listen to a detailed explanation.

Dr. Eric Green, of the National Human Genome Research Institute's Genome Technology Branch, defines DNA sequencing.

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Biology Animation Library

http://vector.cshl.org/Shockwave/cycseq.html

Cycle
Sequencing

PLAY

© 1998 Cold Spring Harbor Laboratory

 

 

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bullet

Interactive Study Guide --

From Gene to Protein: Transcription (By Peter Russell, Reed College) © 1997, Peregrine Publishers, Inc., All Rights Reserved

http://www.biology.com/learning/transcription/intro.html

© 1997, Peregrine Publishers, Inc., All Rights Reserved

Ribosome Song-- www.biology.com/learning/translation/ribosome/ribosome.html

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From Gene to Protein: Translation (Protein Synthesis)© 1997, Peregrine Publishers, Inc., All Rights Reserved

http://www.biology.com/learning/translation/intro.html

Copyright 1998 Genetech, Inc.  All rights reserved

 

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  LabBench

Molecular Biology
by Theresa Knapp Holtzclaw
© 1998, Peregrine Publishers, Inc. All Rights Reserved.
Development Team

Bacterial Transformation

http://www.biology.com/learning/molecular/concept1.html

   

© 1998, Peregrine Publishers, Inc., All Rights Reserved

 

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Investigating the Bacterial Genome
by Thomas Terry, University of Connecticut
© 1996, Peregrine Publishers, Inc., All Rights Reserved
http://www.biology.com/learning/bg/introduction.html

Activity 1: Mapping a Genome  http://www.biology.com/learning/bg/activity1.html

Activity 2: Sorting out Gene Categories
Reading a Gene Map  http://www.biology.com/learning/bg/activity2.html

Activity 3:
Converting Gene Sequences into Protein Sequences
  http://www.biology.com/learning/bg/activity3.html

Activity 4:
Comparing Genomes of Two Organisms
http://www.biology.com/learning/bg/activity4.html

 

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l    TIGR Logo    Description Text

Copyright 1999 TIGR    http://www.tigr.org/index.html

TIGR Microbial Database:
a listing of microbial genomes and chromosomes completed and in progress

  

http://www.tigr.org/tdb/mdb/mdb.htm

 

 

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http://www.biology.com/webquest/webquest.html

Welcome to WebQuests!

Introduce core biological concepts with short single-class period Web exploration activites called WebQuests. Students become engaged in topics such as circulation and respiration as they virtually climb Mt. Everest, for example, or they'll explore plant stimuli by discovering man-eating plants, or explore the fact and fiction of DNA.

WebQuest Activities
CodeQuest: DNA & Coding

http://www.biology.com/webquest/code/intro.html

 

CodeQuest: DNA and Coding 

by Sheila Gilligan, Convent of the Sacred Heart, NY
© 1999, Peregrine Publishers, Inc. All Rights Reserved.

 

 

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